Journal: NPJ Biofilms and Microbiomes

Article Title: In vitro assessment of bacterial supernatants on hypothalamic gene expression: implications for appetite regulation

doi: 10.1038/s41522-025-00820-9

Figure Lengend Snippet: a Overall composition of supernatants analyzing semipolar metabolites. b Overall supernatant composition analyzing SCFAs and BCFA. a , b N = 2 group/media/growth phases ( N = 4 group/media combining both time points or growth phases). Arrow connects CFS samples from the same combination of growth media and bacteria over time, with the origin of the arrow indicating the early stationary phase and the head of the arrow the late stationary phase. CFSs cell-free supernatants, CCSs cell-free conditioned supernatants.

Article Snippet: Supernatants were harvested at both early stationary phase (OD600 of 1.1–1.2 and ~1.3 × 10 9 CFU/ml in B. longum APC1472 case and OD600 1.3–1.4 and ~2.5 × 10 9 CFU/ml in L. reuteri ATCC PTA 6475 case) and late stationary phase (OD600 of 1.4–1.5 and ~2 × 10 9 CFU/ml in B. longum APC1472 case and OD600 1.55–1.7 and ~3×10 9 CFU/ml in L. reuteri ATCC PTA 6475 case).

Techniques: Bacteria

Journal: NPJ Biofilms and Microbiomes

Article Title: In vitro assessment of bacterial supernatants on hypothalamic gene expression: implications for appetite regulation

doi: 10.1038/s41522-025-00820-9

Figure Lengend Snippet: a , b Relative mRNA expression of the orexigenic marker ghrelin ( Ghrl ) and its receptor (growth hormone secretagogue-receptor, Ghsr ), glucagon-like peptide 1 receptor ( Glp1r ), and the receptor type 1 of orexigenic neuropeptide Y ( Npy1r ) after acute exposure of cell-free supernatants (CFSs, white background) and cell-free conditioned supernatants (CCSs, gray background) from B. longum APC1472 or L. reuteri ATCC PTA 6475 to ( a ) embryonic mHypoE-N41 and ( b ) adult mHypoA2/28 hypothalamic mouse cells. CFSs and CCSs from B. longum APC1472 or L. reuteri ATCC PTA 6475 were harvested either at early stationary phase (early) or late stationary phase (late). a , b N = 4 group (bacteria in both growth phases)/media. Data are shown as mean ± SEM. Data are significantly different (* p < 0.05, ** p < 0.01, *** p < 0.001) according to two-way ANOVA followed by Dunnett’s contrasts. CFSs cell-free supernatants, CCSs cell-free conditioned supernatants.

Article Snippet: Supernatants were harvested at both early stationary phase (OD600 of 1.1–1.2 and ~1.3 × 10 9 CFU/ml in B. longum APC1472 case and OD600 1.3–1.4 and ~2.5 × 10 9 CFU/ml in L. reuteri ATCC PTA 6475 case) and late stationary phase (OD600 of 1.4–1.5 and ~2 × 10 9 CFU/ml in B. longum APC1472 case and OD600 1.55–1.7 and ~3×10 9 CFU/ml in L. reuteri ATCC PTA 6475 case).

Techniques: Expressing, Marker, Bacteria

Journal: Molecules

Article Title: Probiotic Yoghurts with Sea Buckthorn, Elderberry, and Sloe Fruit Purees

doi: 10.3390/molecules26082345

Figure Lengend Snippet: Chemical composition of probiotic yoghurts (g/100 g, mean ± SD, n = 4).

Article Snippet: The results of some reports suggested that sea buckthorn berries may improve the viability and serve as an efficient immobilization carrier to protect probiotic cells (e.g., L. casei ATCC 393) in dairy matrices [ , , ].

Techniques:

Journal: Molecules

Article Title: Probiotic Yoghurts with Sea Buckthorn, Elderberry, and Sloe Fruit Purees

doi: 10.3390/molecules26082345

Figure Lengend Snippet: Antioxidant parameters, acidity, number of starter bacteria, and concentrations of aromatic compounds in the probiotic yoghurts during storage (mean ± SD, n = 4).

Article Snippet: The results of some reports suggested that sea buckthorn berries may improve the viability and serve as an efficient immobilization carrier to protect probiotic cells (e.g., L. casei ATCC 393) in dairy matrices [ , , ].

Techniques: Bacteria

Journal: Molecules

Article Title: Probiotic Yoghurts with Sea Buckthorn, Elderberry, and Sloe Fruit Purees

doi: 10.3390/molecules26082345

Figure Lengend Snippet: Textural and color parameters and syneresis of the probiotic yoghurts (mean ± SD, n = 4).

Article Snippet: The results of some reports suggested that sea buckthorn berries may improve the viability and serve as an efficient immobilization carrier to protect probiotic cells (e.g., L. casei ATCC 393) in dairy matrices [ , , ].

Techniques: Viscosity

Journal:

Article Title: Differentiation of Lactobacillus Species by Molecular Typing

doi:

Figure Lengend Snippet: Molecular typing of Lactobacillus isolates with respect to ATCC typed strains

Article Snippet: The nomenclature used here for the type and reference strains was that given by ATCC and NCFB prior to 1993, and that used for the clinical and stock isolates was the same as that reported in publications by our group ( 23 , 24 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Original nomenclature Ribotype with Bcl I Chromotype with Bcl I L. rhamnosus ATCC 7469 T A 1.1 L. rhamnosus ATCC 11443 A 1.1 L. casei subsp. rhamnosus 81 A 1.1 L. casei subsp. rhamnosus 76 A 1.2 L. rhamnosus ATCC 8530 A 1.3 L. casei subsp. rhamnosus GR-1 A 1.4 L. casei subsp. rhamnosus 36 A 1.4 Vaginal isolate w A 1.4 Vaginal isolate g A 1.4 L. rhamnosus NCFB 2964 A 1.5 L. casei subsp. rhamnosus RC-17 A 1.6 L. fermentum A60 A 1.6 L. plantarum RC-6 A 1.7 L. rhamnosus ATCC 21052 A 2.1 C1-A A 2.1 C3-A A 2.1 C3-B A 2.1 C5-A A 2.1 C7-A A 2.1 C7-B A 2.1 L. casei subsp. casei ATCC 393 B 10.1 L. casei subsp. casei ATCC 4940 B1 11.1 L. paracasei subsp. paracasei ATCC 25302 T C 20.1 L. casei subsp. casei ATCC 334 C 20.2 L. casei subsp. casei ATCC 4007 C1 21.1 L. casei subsp. casei ATCC 4913 C2 22.1 L. casei subsp. casei 55 C2 23.1 L. paracasei subsp. paracasei ATCC 25303 C2 24.1 L. casei subsp. rhamnosus 43 C3 25.1 L. casei 8 C3 25.1 L. casei 65 C3 25.1 L. plantarum ATCC 14917 T D 30.1 L. plantarum UH 2153 D 30.1 L. plantarum ATCC 10012 D 30.2 L. plantarum ATCC 11974 D1 31.1 L. plantarum ATCC 14431 D2 32.1 L. plantarum ATCC 8014 D3 33.1 L. plantarum 260 D3 33.1 L. plantarum RC-20 D4 34.1 L. acidophilus 75 D5 34.2 US2-A D6 35.1 US2-B D6 35.1 US2-C D6 35.1 US3-A D7 35.2 US3-B D7 35.2 US6-A D8 36.1 US6-B D8 36.1 US6-C D8 36.1 C4-A D8 36.1 C4-B D8 36.1 US8-A D9 37.1 L. fermentum ATCC 14931 T E 40.1 L. fermentum ATCC 23271 E1 40.2 L. fermentum ATCC 8289 E2 40.3 L. fermentum ATCC 9338 E2 40.3 L. fermentum ATCC 11739 E3 41.1 L. fermentum ATCC 14932 E4 42.1 L. fermentum B-54 E5 43.1 L. acidophilus RC-14 E5 43.1 L. acidophilus RC-14 (subcultured 20 times) E5 43.1 L. acidophilus ATCC 4356 T F 50.1 US4 F 50.2 L. acidophilus T-13 F1 51.1 L. jensenii ATCC 25258 T G 60.1 Open in a separate window Molecular typing of Lactobacillus isolates with respect to ATCC typed strains Nine health food products were examined.

Techniques:

Journal:

Article Title: Differentiation of Lactobacillus Species by Molecular Typing

doi:

Figure Lengend Snippet: Autoradiogram of Southern blot of BclI-digested DNA after hybridization with 32P-labelled rRNA from E. coli. Lanes 1 to 12 represent ribotype groups A to F. Lane 1, L. rhamnosus ATCC 7469T; lane 2, L. casei subsp. casei ATCC 393; lane 3, L. paracasei subsp. paracasei ATCC 25302T; lane 4, L. casei subsp. casei ATCC 4007; lane 5, L. paracasei subsp. paracasei ATCC 25303; lane 6, L. casei 8; lane 7, L. plantarum ATCC 14917T; lane 8, L. plantarum ATCC 11974; lane 9, L. plantarum ATCC 14431; lane 10, L. fermentum B-54; lane 11, L. acidophilus ATCC 4356T; lane 12, L. acidophilus T-13.

Article Snippet: The nomenclature used here for the type and reference strains was that given by ATCC and NCFB prior to 1993, and that used for the clinical and stock isolates was the same as that reported in publications by our group ( 23 , 24 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Original nomenclature Ribotype with Bcl I Chromotype with Bcl I L. rhamnosus ATCC 7469 T A 1.1 L. rhamnosus ATCC 11443 A 1.1 L. casei subsp. rhamnosus 81 A 1.1 L. casei subsp. rhamnosus 76 A 1.2 L. rhamnosus ATCC 8530 A 1.3 L. casei subsp. rhamnosus GR-1 A 1.4 L. casei subsp. rhamnosus 36 A 1.4 Vaginal isolate w A 1.4 Vaginal isolate g A 1.4 L. rhamnosus NCFB 2964 A 1.5 L. casei subsp. rhamnosus RC-17 A 1.6 L. fermentum A60 A 1.6 L. plantarum RC-6 A 1.7 L. rhamnosus ATCC 21052 A 2.1 C1-A A 2.1 C3-A A 2.1 C3-B A 2.1 C5-A A 2.1 C7-A A 2.1 C7-B A 2.1 L. casei subsp. casei ATCC 393 B 10.1 L. casei subsp. casei ATCC 4940 B1 11.1 L. paracasei subsp. paracasei ATCC 25302 T C 20.1 L. casei subsp. casei ATCC 334 C 20.2 L. casei subsp. casei ATCC 4007 C1 21.1 L. casei subsp. casei ATCC 4913 C2 22.1 L. casei subsp. casei 55 C2 23.1 L. paracasei subsp. paracasei ATCC 25303 C2 24.1 L. casei subsp. rhamnosus 43 C3 25.1 L. casei 8 C3 25.1 L. casei 65 C3 25.1 L. plantarum ATCC 14917 T D 30.1 L. plantarum UH 2153 D 30.1 L. plantarum ATCC 10012 D 30.2 L. plantarum ATCC 11974 D1 31.1 L. plantarum ATCC 14431 D2 32.1 L. plantarum ATCC 8014 D3 33.1 L. plantarum 260 D3 33.1 L. plantarum RC-20 D4 34.1 L. acidophilus 75 D5 34.2 US2-A D6 35.1 US2-B D6 35.1 US2-C D6 35.1 US3-A D7 35.2 US3-B D7 35.2 US6-A D8 36.1 US6-B D8 36.1 US6-C D8 36.1 C4-A D8 36.1 C4-B D8 36.1 US8-A D9 37.1 L. fermentum ATCC 14931 T E 40.1 L. fermentum ATCC 23271 E1 40.2 L. fermentum ATCC 8289 E2 40.3 L. fermentum ATCC 9338 E2 40.3 L. fermentum ATCC 11739 E3 41.1 L. fermentum ATCC 14932 E4 42.1 L. fermentum B-54 E5 43.1 L. acidophilus RC-14 E5 43.1 L. acidophilus RC-14 (subcultured 20 times) E5 43.1 L. acidophilus ATCC 4356 T F 50.1 US4 F 50.2 L. acidophilus T-13 F1 51.1 L. jensenii ATCC 25258 T G 60.1 Open in a separate window Molecular typing of Lactobacillus isolates with respect to ATCC typed strains Nine health food products were examined.

Techniques: Southern Blot, Hybridization